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KMID : 0624620130460070358
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BMB Reports
2013 Volume.46 No. 7 p.358 ~ p.363
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A novel pattern recognition protein of the Chinese oak silkmoth, Antheraea pernyi, is involved in the pro-PO activating system
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Wang Xialu
Zhang Jinghai
Chen Ying
Ma Youlei
Zou Wenjun
Ding Guoyuan
Li Wei
Zhao Mingyi
Wu Chunfu
Zhang Rong
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Abstract
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In this paper, we firstly reported a C-type lectin cDNA clone of 1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR and RACE techniques. The full-length cDNA contains an open reading frame encoding 308 amino acid residues which has two different carbohydrate-recognition domains (CRDs) arranged in tandem. To investigate the biological activities in the innate immunity, recombinant Ap-CTL was expressed in E. coli with a 6-histidine at the amino-terminus (Ap-rCTL). Besides acted as a broad-spectrum recognition protein binding to a wide range of PAMPs and microorganisms, Ap-rCTL also had the ability to recognize and trigger the agglutination of bacteria and fungi. In the proPO activation assay, Ap-rCTL specifically restored the PO activity of hemolymph blocked by anti- Ap-rCTL antibody in the presence of different PAMPs or microorganisms. In summary, Ap-rCTL plays an important role in insect innate immunity as an pattern recognition protein.
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KEYWORD
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Antheraea pernyi, C-type lectin, Innate immunity, Pattern recognition protein, Pro-PO activation
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